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US 2021/0221870 A1Published application
Cell culture media containing combinations of proteins
AssigneeVentria Bioscience
InventorsSteven Clyde Pettit, Mary Ann Michelle Fernandez Santos, Ning Huang
Life of this patent
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Abstract
The present invention relates to methods of enhancing transfection in cell culture media supplemented with a plant-produced recombinant mammalian transferrin supplement, as well as kits, and methods of using the supplemented cell culture media to improve growth characteristics of cultured cells for transfection.
Claims16
A method of enhancing transfection efficiency or viability of a cell during transfection, said method comprising:
- a) transfecting a cell with a nucleic acid molecule in a cell culture media supplemented with a plant-produced recombinant mammalian transferrin related protein, wherein said transfection efficiency or cell viability is enhanced relative to a culture without said plant-produced recombinant mammalian transferrin related protein supplement.
A kit for enhancing transfection efficiency or viability of a cell during transfection, said kit comprising:
- (a) one or more reagents or devices for transfection;
- (b) a plant-produced recombinant mammalian transferrin related protein supplement;
- and (c) instructions for transfecting a cell with a nucleic acid molecule in a cell culture media supplemented with said plant-produced recombinant mammalian transferrin related protein supplement.
Dependencies are inferred from the claim text.