A synthetic polypeptide epitope based vaccine composition
AssigneeBharat Biotech International Limited
InventorAmit RAYCHOUDHURI
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Abstract
Conserved epitopes selected from EV71 and CVA16, the two major causative agents of Hand Foot and Mouth Disease has been used to develop sub-unit bivalent vaccine antigen construct. The said vaccine described in this invention is capable to provide cross-protection to diverse EV71 and CVA16 infection causing strains. Further disclosed are the expression of the multi-epitope vaccine antigen coding gene and the purification process involved thereof. This present invention also discloses vaccine formulations against Hand Foot and Mouth Disease and other enterovirus infections comprising the recombinant vaccine antigen construct of the present invention.
Claims9
A vaccine composition for prophylaxis against Hand Foot and Mouth Disease caused by EV71 and CA16 comprising:
- a. vaccine antigen, the said vaccine antigen is a synthetic construct selected from the recombinant protein sequences as represented by SEQ ID No. 2 (named as MEV1) and SEQ ID No. 4 (named as MEV2);
- b. adjuvants;
- c. stabilizers;
- and
- d. a physiologically acceptable buffer selected from phosphate and citrate;
- wherein the said vaccine formulation is stable for at least 2 years at 5±3° C. and up to 2 weeks at 37° C.
A multi-epitope synthetic protein sequence as disclosed in SEQ ID No. 2 with at least 92%-96% purity.
A multi-epitope synthetic protein sequence as disclosed in SEQ ID no. 4 with at least 96% purity.
Dependencies are inferred from the claim text.