Disposable electrochemical biosensor based on NAD(P)-dependent dehydrogenase and diaphorase
AssigneeNova Biomedical Corporation
InventorsJianhong Pei, Joseph Bedard, Aaron Tram, Samantha Mosley, Chung Chang Young
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Abstract
A disposable biosensor that includes a sensing surface having at least a working electrode portion and a reference electrode portion, a first reagent disposed on the working electrode portion containing a NAD(P)-dependent dehydrogenase, NAD(P)+, diaphorase, and an oxidized form of a redox mediator forming a working electrode, and a reference electrode material disposed on the reference electrode portion forming a reference electrode.
Claims18
A disposable biosensor comprising:
- a sensing surface having at least a working electrode portion and a reference electrode portion;
- a first reagent disposed on the working electrode portion containing a NAD(P)-dependent dehydrogenase, NAD(P)+, diaphorase, and an oxidized form of a redox mediator forming a working electrode;
- and
- a reference electrode material disposed on the reference electrode portion forming a reference electrode.
A method of making a disposable biosensor based on NADP-dependent dehydrogenase and diaphorase, the method comprising:
- providing a sensing surface having at least a working electrode portion and a reference electrode portion;
- disposing a first reagent on the working electrode portion, the first reagent containing a NAD(P)-dependent dehydrogenase, NADP+, diaphorase, and an oxidized form of a redox mediator and drying the first reagent forming a first electrode matrix creating a working electrode;
- disposing a reference electrode material on the reference electrode portion forming a reference electrode wherein the reference electrode material is one of Ag—AgCl or a reference electrode reagent containing at least a chemically oxidizing reagent selected from the group consisting of a reduced form of a redox mediator, an oxidized form of a redox mediator and a mixture of a reduced form and an oxidized form of a redox mediator and drying the reference electrode reagent forming a reference electrode matrix.
Dependencies are inferred from the claim text.