Image processing techniques using digital holographic microscopy

A non-transitory device-readable storage medium including instructions that, when executed by processing circuitry, cause the processing circuitry to perform operations, the operations comprising:

• calculating, for each of a predetermined set of depths, a free space impulse response at double precision, each of the depths being determined from a reference plane;
• capturing, at an image sensor, a hologram after calculating the free space impulse responses, the reference plane associated with the image sensor;
• calculating, at single precision, a forward Fast Fourier Transform (FFT) of the hologram at each of the depths:
• multiplying, at each of the depths, a forward FFT of the free space impulse response at the depth with the forward FFT of the hologram at the depth to form a two-dimensional (2D) matrix;
• calculating, for each depth, an inverse FFT of the 2D matrix to form an image slice at the depth;
• and
• combining the image slice from each of the depths to reconstruct the hologram in at least one of a combined 2D or 3D array.

A system comprising:

• digital holographic microscopy imaging equipment comprising:
• a light source configured to emit light toward an object,
• an opaque material that is opaque to the light, the opaque material having a pinhole arranged to pass the light therethrough toward the object, and
• an image sensor disposed on an opposite side of the object as the opaque material, the image sensor configured to capture the light after scattering by the object without a lens being disposed between the light source and the image sensor;
• and
• computing components configured to:
• calculate, for each of a predetermined set of depths, a free space impulse response at double precision;
• receive holographic data of a hologram of the object captured by the image sensor after calculating the free space impulse responses;
• at each of the depths, calculate, at single precision, a forward Fast Fourier Transform (FFT) of the hologram;
• at each of the depths, multiply a forward FFT of the free space impulse response at the depth with the forward FFT of the hologram at the depth to form a two-dimensional (2D) matrix;
• at each of the depths, calculate an inverse FFT of the 2D matrix to form an image slice;
• and
• combine the image slices of each of the depths to reconstruct the hologram of the object.