US 2021/0214745 A1 · SYNTHETIC GENES

Abstract

The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.

Claims18

Dependent claims

A synthetic DNA sequence for expressing a protein of interest in maize cells which comprises:

a) a codon-optimized DNA sequence encoding the protein of interest,
b) at least one polyadenylation signal sequence chosen from the group consisting of Class I and Class II wherein;
Class I is chosen from the group consisting of AATAAA, AATAAT, AACCAA, ATATAA, AATCAA, ATACTA, ATAAAA, ATGAAA, AAGCAT, ATTAAT, ATACAT, AAAATA, ATTAAA, AATTAA, AATACA, and CATAAA;
and
Class II is chosen from the group consisting of ATATAT, TTGTTT, TTTTGT, TGTTTT, TATATA, TATTT, TrTTT, ATTTr, TTATTT, TTTATT, TAATAA, ATTTAT, TATATT, TrTTAT, ATATTT, TATTAT, TGTTTG, TTATAT, TGTAAT, and AAATAA;
and
wherein said codon-optimized DNA sequence contains at least one polyadenylation signal sequence from Class II and wherein said synthetic DNA sequence contains fewer Class II polyadenylation signal sequences than the protein's native DNA sequence and contains the same number of class I polyadenylation signal sequences compared to said native DNA sequence.

4 more elements

Dependencies are inferred from the claim text.

Synthetic genes

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Synthetic genes

Life of this patent

Abstract

Claims18