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US 2021/0214729 A1Published application
Substitution of the messenger rna cap with two rna sequences introduced at the 5-prime end thereof
OwnerJerome LEMOINEinventor
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Abstract
The invention relates to a messenger ribonucleic acid (mRNA) molecule lacking a cap molecule, for which the cost of in vitro transcription synthesis is greatly reduced, comprising, from 5′ to 3′, at least one copy of a GUCAGRYC(N7-19)GCCA(N12-19)UGCNRYCUG consensus sequence which is resistant to the Xrn1 exoribonuclease, a copy of an internal ribosome entry site (IRES) RNA sequence, and an open reading frame.
Claims20
A messenger ribonucleic acid (mRNA) molecule lacking a cap molecule comprising from 5′ to 3′:
- a 5′-UTR region comprising at least one copy of a consensus sequence GUCAGRYC(N7-19)GCCA(N12-19)UGCNRYCUG (xrRNA);
- a copy of an internal ribosome entry site (IRES) RNA sequence;
- and
- at least one open reading frame.
1 more element
Dependencies are inferred from the claim text.