Methods of reprogramming somatic cells and materials related thereto
AssigneeCity of Hope
InventorsArthur D. RIGGS, Avinash C. SRIVASTAVA, Timothy R. O'CONNOR
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Abstract
Disclosed herein are methods for reprogramming a somatic cell into a pluripotent stem cell by contacting the somatic cell with one or more antifolate agents, with or without methionine, in vitro for a period of time sufficient to reprogramming the somatic cell and selecting and growing the cells that express one or more stem cell markers. Also disclosed are induced pluripotent stem cells obtained from somatic cells.
Claims24
A method of reprogramming somatic cells into pluripotent stem cells, comprising:
- contacting one or more somatic cells with one or more antifolate agents in vitro for a period of time sufficient to induce reprogramming;
- selecting cells expressing one or more stem cell markers;
- and
- growing the selected cells to obtain the induced pluripotent stem cells (iPSCs).
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A pluripotent stem cell obtained by reprogramming a somatic cell, the reprogramming comprises:
- contacting the somatic cell with one or more antifolate agents in vitro for a period of time sufficient to induce reprogramming;
- selecting cells expressing one or more stem cell markers;
- and
- growing the selected cells to obtain the induced pluripotent stem cell, wherein the obtained pluripotent stem cell expresses one or more of the markers including OCT4, SOX2, SSEA-4, Nanog, and TRA 1-60.
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Dependencies are inferred from the claim text.