Method for producing l-methionine using a bacterium

A method for producing L-methionine comprising:

• (i) cultivating in a culture medium a bacterium which has an ability to produce L-methionine to produce and accumulate the L-methionine in the culture medium or cells of the bacterium, or both, and
• (ii) collecting the L-methionine from the culture medium or the cells, or both,
• wherein said bacterium has been modified to overexpress a 1st gene and a 2nd gene,
• wherein said 1st gene is selected from the group consisting of:
• (1A) a DNA comprising the nucleotide sequence shown in SEQ ID NO:
• 1,
• (1B) a DNA encoding a protein comprising the amino acid sequence shown in SEQ ID NO:
• 2,
• (1C) a DNA encoding a protein comprising the amino acid sequence shown in SEQ ID NO:
• 2, but wherein said amino acid sequence includes substitution, deletion, insertion, and/or addition of 1 to 30 amino acid residues, and wherein said DNA comprises a property that if the DNA is overexpressed in the bacterium, the amount of L-methionine produced by the bacterium is increased as compared with that observed for a non-modified strain,
• (1D) a DNA encoding a protein comprising an amino acid sequence having an identity of not less than 85% with respect to the entire amino acid sequence shown in SEQ ID NO:
• 2, and wherein said DNA comprises a property that if the DNA is overexpressed in the bacterium, the amount of L-methionine produced by the bacterium is increased as compared with that observed for a non-modified strain,
• (1E) a DNA comprising a nucleotide sequence that is able to hybridize under stringent conditions with a nucleotide sequence complementary to the sequence shown in SEQ ID NO:
• 1, and wherein said DNA comprises a property that if the DNA is overexpressed in the bacterium, the amount of L-methionine produced by the bacterium is increased as compared with that observed for a non-modified strain,
• (1F) a DNA comprising a nucleotide sequence having an identity of not less than 85% with respect to the entire nucleotide sequence shown in SEQ ID NO:
• 1, and wherein said DNA comprises a property that if the DNA is overexpressed in the bacterium, the amount of L-methionine produced by the bacterium is increased as compared with that observed for a non-modified strain, and
• (1G) a DNA comprising a variant nucleotide sequence of SEQ ID NO:
• 1 due to the degeneracy of the genetic code, and
• wherein said 2nd gene is selected from the group consisting of:
• (2A) a DNA comprising the nucleotide sequence shown in SEQ ID NO:
• 3,
• (2B) a DNA encoding a protein comprising the amino acid sequence shown in SEQ ID NO:
• 4,
• (2C) a DNA encoding a protein comprising the amino acid sequence shown in SEQ ID NO:
• 4, but wherein said amino acid sequence includes substitution, deletion, insertion, and/or addition of 1 to 30 amino acid residues, and wherein said DNA comprises a property that if the DNA is overexpressed in the bacterium, the amount of L-methionine produced by the bacterium is increased as compared with that observed for a non-modified strain,
• (2D) a DNA encoding a protein comprising an amino acid sequence having an identity of not less than 85% with respect to the entire amino acid sequence shown in SEQ ID NO:
• 4, and wherein said DNA comprises a property that if the DNA is overexpressed in the bacterium, the amount of L-methionine produced by the bacterium is increased as compared with that observed for a non-modified strain,
• (2E) a DNA comprising a nucleotide sequence that is able to hybridize under stringent conditions with a nucleotide sequence complementary to the sequence shown in SEQ ID NO:
• 3, and wherein said DNA comprises a property that if the DNA is overexpressed in the bacterium, the amount of L-methionine produced by the bacterium is increased as compared with that observed for a non-modified strain,
• (2F) a DNA comprising a nucleotide sequence having an identity of not less than 85% with respect to the entire nucleotide sequence shown in SEQ ID NO:
• 3, and wherein said DNA comprises a property that if the DNA is overexpressed in the bacterium, the amount of L-methionine produced by the bacterium is increased as compared with that observed for a non-modified strain, and
• (2G) a DNA comprising a variant nucleotide sequence of SEQ ID NO:
• 3 due to the degeneracy of the genetic code.