Bladder cell formation control agent and plant body into which bladder cell formation control agent has been introduced

A plant body having enhanced expression of a protein having an epidermal bladder cell formation action, which has an amino acid sequence of any one of the following items (1) to (4):

• (1) an amino acid sequence set forth in SEQ ID NO:
• 3;
• (2) an amino acid sequence that has 1 to 20 amino acids substituted, deleted, inserted, and/or added in the amino acid sequence set forth in SEQ ID NO:
• 3, and that has an epidermal bladder cell formation action substantially equivalent to that of the amino acid sequence set forth in SEQ ID NO:
• 3;
• (3) an amino acid sequence that has 90% or more homology to the amino acid sequence set forth in SEQ ID NO:
• 3, and that has an epidermal bladder cell formation action substantially equivalent to that of the amino acid sequence set forth in SEQ ID NO:
• 3;
• and
• (4) the amino acid sequence of at least one of the items (1) to (3) including an amino acid sequence set forth in SEQ ID NO:
• 5, which is a single-base-substituted WD domain.

A method of forming epidermal bladder cells in a plant body, including introducing one of a gene shown in any one of the following items (1) to (8) and a vector carrying the gene or a protein having an epidermal bladder cell formation action, which has an amino acid sequence of any one of the following items (9) to (12), into a plant body:

• (1) a gene encoding a polypeptide having an amino acid sequence set forth in SEQ ID NO:
• 3;
• (2) a gene encoding a polypeptide that has 1 to 20 amino acids substituted, deleted, inserted, and/or added in the amino acid sequence set forth in SEQ ID NO:
• 3, and that has an epidermal bladder cell formation action substantially equivalent to that of the amino acid sequence set forth in SEQ ID NO:
• 3;
• (3) a gene encoding a polypeptide that has 90% or more homology to the amino acid sequence set forth in SEQ ID NO:
• 3, and that has an epidermal bladder cell formation action substantially equivalent to that of the amino acid sequence set forth in SEQ ID NO:
• 3;
• (4) a gene formed of DNA having a base sequence set forth in SEQ ID NO:
• 4;
• (5) a gene formed of DNA that hybridizes with DNA having a base sequence complementary to DNA having the base sequence set forth in SEQ ID NO:
• 4 under stringent conditions, and that encodes a polypeptide having an epidermal bladder cell formation action;
• (6) a gene formed of DNA having a 1- to 50-base sequence substituted, deleted, inserted, and/or added in DNA having the base sequence set forth in SEQ ID NO:
• 4;
• (7) a gene formed of DNA having 90% or more homology to DNA having the base sequence set forth in SEQ ID NO:
• 4;
• (8) the gene of at least one of the items (1) to (7) including a gene encoding a polypeptide having an amino acid sequence set forth in SEQ ID NO:
• 5, which is a single-base-substituted WD domain;
• (9) an amino acid sequence set forth in SEQ ID NO:
• 3;
• (10) an amino acid sequence that has 1 to 20 amino acids substituted, deleted, inserted, and/or added in the amino acid sequence set forth in SEQ ID NO:
• 3, and that has an epidermal bladder cell formation action substantially equivalent to that of the amino acid sequence set forth in SEQ ID NO:
• 3;
• (11) an amino acid sequence that has 90% or more homology to the amino acid sequence set forth in SEQ ID NO:
• 3, and that has an epidermal bladder cell formation action substantially equivalent to that of the amino acid sequence set forth in SEQ ID NO:
• 3;
• and
• (12) the amino acid sequence of at least one of the items (9) to (11) including an amino acid sequence set forth in SEQ ID NO:
• 5, which is a single-base-substituted WD domain.

A method of suppressing epidermal bladder cell formation in a plant body, including introducing one of a gene shown in any one of the following items (1) to (8) and a vector carrying the gene or a protein having an epidermal bladder cell formation suppression action, which has an amino acid sequence of any one of the following items (9) to (12), into a plant body:

• (1) a gene encoding a polypeptide having an amino acid sequence set forth in SEQ ID NO:
• 1;
• (2) a gene encoding a polypeptide that has 1 to 20 amino acids substituted, deleted, inserted, and/or added in the amino acid sequence set forth in SEQ ID NO:
• 1, and that has an epidermal bladder cell formation suppression action substantially equivalent to that of the amino acid sequence set forth in SEQ ID NO:
• 1;
• (3) a gene encoding a polypeptide that has 90% or more homology to the amino acid sequence set forth in SEQ ID NO:
• 1, and that has an epidermal bladder cell formation suppression action substantially equivalent to that of the amino acid sequence set forth in SEQ ID NO:
• 1;
• (4) a gene formed of DNA having a base sequence set forth in SEQ ID NO:
• 2;
• (5) a gene formed of DNA that hybridizes with DNA having a base sequence complementary to DNA having the base sequence set forth in SEQ ID NO:
• 2 under stringent conditions, and that encodes a polypeptide having an epidermal bladder cell formation suppression action;
• (6) a gene formed of DNA having a 1- to 50-base sequence substituted, deleted, inserted, and/or added in DNA having the base sequence set forth in SEQ ID NO:
• 2;
• (7) a gene formed of DNA having 90% or more homology to DNA having the base sequence set forth in SEQ ID NO:
• 2;
• (8) the gene of at least one of the items (1) to (7) including a gene encoding a polypeptide having an amino acid sequence set forth in SEQ ID NO:
• 6, which is a single-base-substituted WD domain;
• (9) an amino acid sequence set forth in SEQ ID NO:
• 1;
• (10) an amino acid sequence that has 1 to 20 amino acids substituted, deleted, inserted, and/or added in the amino acid sequence set forth in SEQ ID NO:
• 1, and that has an epidermal bladder cell formation suppression action substantially equivalent to that of the amino acid sequence set forth in SEQ ID NO:
• 1;
• (11) an amino acid sequence that has 90% or more homology to the amino acid sequence set forth in SEQ ID NO:
• 1, and that has an epidermal bladder cell formation suppression action substantially equivalent to that of the amino acid sequence set forth in SEQ ID NO:
• 1;
• and
• (12) the amino acid sequence of at least one of the items (9) to (11) including an amino acid sequence set forth in SEQ ID NO:
• 6, which is a single-base-substituted WD domain.