Strain and Method for Producing Rosmarinic Acid
AssigneeHong-Taoism Research Institute of analytical science and technology LTD., Shaanxi Province
InventorsYujie CAI, Yi YAN, Yanrui DING, Yajun BAI, Xiaohui ZHENG
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Abstract
The disclosure discloses a strain and method for producing rosmarinic acid, and belongs to the technical field of bioengineering. The disclosure constructs a recombinant cell or a combination of recombinant cells expressing 4-coumarate: CoA ligase, rosmarinic acid synthase, polyphosphate kinase 2-I (PPK2-I) and polyphosphate kinase 2-II (PPK2-II), and utilizes the recombinant cell or the combination of recombinant cells to catalyze Danshensu and caffeic acid for synthesizing rosmarinic acid. The disclosure has good industrial application prospects.
Claims14
A recombinant microbial cell, wherein the recombinant microbial cell is capable of synthesizing rosmarinic acid using phenolic acids as substrates, and the recombinant microbial cell expresses 4-coumarate:
- CoA ligase, rosmarinic acid synthase, polyphosphate kinase 2-I (PPK2-I) and polyphosphate kinase 2-II (PPK2-II).
A combination of recombinant cells, wherein the combination of recombinant cells is capable of synthesizing rosmarinic acid using phenolic acids as substrates;
- the combination of recombinant cells comprises recombinant cells expressing one or more of 4-coumarate:
- CoA ligase, rosmarinic acid synthase, polyphosphate kinase 2-II (PPK2-II) and polyphosphate kinase 2-I (PPK2-I) separately, and each recombinant cell does not repeatedly express one of 4-coumarate:
- CoA ligase, rosmarinic acid synthase, PPK2-II, and PPK2-I expressed by other recombinant cells.
Dependencies are inferred from the claim text.