Method for analyzing behavior of cell, and use thereof

A method for evaluating a cell, the method comprising:

• detecting positions of a plurality of cells for every frame of time-lapse images, while determining whether a candidate region extracted from the frame is a cell region by using a dictionary containing image data of cell nuclei;
• tracking position of each cell by using a state space model using position of a most adjacent cell within a predetermined distance from a predicted position as observation data;
• and
• calculating speed information of each cell on the basis of tracking information obtained in the tracking step.

A method for producing a cultured tissue, the method comprising:

• culturing cells obtained from an object to prepare a cultured tissue for transplantation;
• and
• evaluating the prepared cultured tissue by using locomotion information of cells contained in the cultured tissue as an index;
• and wherein the evaluation step comprises:
• detecting positions of a plurality of cells for every frame of time-lapse images of all or a part of the cultured tissue;
• tracking each cell by using a Kalman filter of which observation data is a position of a most adjacent cell within a predetermined distance from a predicted position of the cell at present time, which is the position of the cell in the frame of previous time point;
• and
• calculating locomotion information of each cell on the basis of tracking information obtained in the tracking step.