Spr-based bridging assay format for determining the biological activity of multivalent, multispecific molecules
AssigneeHoffmann-La Roche Inc.
InventorsApollon Papadimitriou, Joerg Thomas Regula, Hubert Kettenberger, Joerg Moelleken, Christian Gassner
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Abstract
Herein is reported the use of a binding assay of a bivalent, bispecific antibody that has the smaller kD value (dissociation constant) for the interaction with its antigen for the immobilization of the bivalent, bispecific antibody to a solid surface for the determination of the biological activity of the bivalent, bispecific antibody.
Claims12
Use of the simultaneous binding of a bivalent, bispecific antibody to its first and second antigen in a surface plasmon resonance or ELISA method, whereby the first antigen is immobilized as capture reagent on a solid surface, and whereby the bivalent, bispecific antibody has a kD value for the interaction with the first antigen that is smaller than the kD value for the interaction with the second antigen, for reducing the interference of functional multimeric forms of the bivalent, bispecific antibody in the surface plasmon resonance or ELISA method.
A method for determining the presence of functional multimers of a bivalent, bispecific antibody in a sample, comprising the step of
- comparing the binding signal determined for the bivalent, bispecific antibody using an assay wherein a first antigen is immobilized and used for the capture of the bivalent, bispecific antibody with the binding signal determined for the bivalent, bispecific antibody using the same assay wherein the second antigen is immobilized and used for the capture of the bivalent, bispecific antibody,
- whereby the presence of functional multimers of the bivalent, bispecific antibody is determined if the determined binding signal differ by more than the standard deviation of the performed assay.
Dependencies are inferred from the claim text.