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US 2021/0199658 A1Published application
Detection of lipase activity in honey bees
OwnerYanping Chen, Matthew C. Heerman +2inventors
InventorsYanping Chen, Matthew C. Heerman, Steven C. Cook, Jay D. Evans
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Abstract
This disclosure relates generally to novel kits for measuring insect health, and to methods of making and using such compositions. More specifically, the invention relates to novel kits for a rapid and high-throughput measurement of lipase activity levels in insects, and the correlation of the measured lipase activity levels with insect stress.
Claims22
A kit comprising:
- a fluorogenic triglyceride comprising a dye and a quencher;
- and
- a substrate reaction buffer comprising Zwittergent detergent in Phosphate Buffered Saline (PBS).
A method for determining lipase activity levels in an insect biological sample, the method comprising:
- mixing an insect biological sample in a buffer comprising BSA, Zwittergent detergent, and PBS;
- with a working solution comprising a fluorogenic triglyceride comprising a dye and a quencher and Zwittergent detergent in PBS;
- and
- measuring the emitted fluorescence;
- wherein the measured emitted fluorescence is an indication of the lipase activity in the insect biological sample.
2 more elements
Dependencies are inferred from the claim text.