Immunogenic peptides and use thereof
OwnerPierre-Alain Rubbo, Edouard Tuailloninventors
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Abstract
The invention relates to an in vitro method of screening immunogenic peptides of interest, capable of recognizing antibodies originating from the serum of individuals suffering from active tuberculosis, said method comprising:—bringing into contact of peptides of the serum originating from patients suffering from active tuberculosis—detecting the formation of immune complexes in the preceding step, and—selecting peptides of interest for which the value of a ratio R is greater than or equal to 1.5, the ratio R being the measurement value of the formation of immune complexes to the measurement value obtained from the control sample.
Claims11
An in vitro method of screening at least one immunogenic peptide of interest capable of recognizing at least one antibody originating from the serum of individuals suffering from active tuberculosis, said at least one immunogenic peptide being a hydrophilic peptide originating from a hydrophobic protein, said hydrophobic protein being a wall protein, or secreted from bacteria from the Mycobacterium genus, said hydrophobic protein having a lipolytic activity,
- said method comprising the following steps:
- bringing into contact of at least one hydrophilic peptide originating from at least one hydrophobic peptide with
- successively at least two independent pools of serums originating from patients suffering from confirmed active tuberculosis, to allow the formation of immune complexes between said antibodies and said peptides to be screened, and
- at least one control sample originating from an individual not suffering from tuberculosis,
- detecting the formation of immune complexes in the previous step,
- conducting a first selection of the peptides of interest for which the value of a ratio R is greater than or equal to 1.5 for at least one of the pools of independent serums originating from patients suffering from confirmed active tuberculosis,
- the ratio R being the normalized measurement value of the formation of immune complexes to the normalized measurement value obtained from the sample originating from a healthy individual.
A hydrophilic peptide, comprising, or consisting of, 15 to 25 amino acids, originating from a hydrophobic protein, said hydrophobic protein being a wall protein or secreted from bacteria of the Mycobacterium genus, said hydrophobic protein having a lipolytic activity.
The hydrophilic peptide according to claim 6, said peptide being represented by any one of the following sequences:
- SEQ ID NO:
- 1 to SEQ ID NO. 30, in particular by any one of the following sequences:
- SEQ ID NO:
- 1 to SEQ ID NO:
- 5.
(canceled)
Dependencies are inferred from the claim text.