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US 2021/0198754 A1Published application
Massively parallel single cell analysis
AssigneeBecton, Dickinson and Company
InventorsChristina Fan, Stephen P.A. Fodor, Glenn Fu, Geoffrey Richard Facer, Julie Wilhelmy
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Abstract
The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).
Claims26
A instrument system for performing multiplexed single cell assay, comprising:
- a microtiter plate or a microfluidic chip;
- and
- a plurality of particles each associated with a plurality of oligonucleotides, wherein each of the plurality of oligonucleotides comprises a cellular label sequence, a molecular label sequence, and a target-binding region, wherein the cellular label sequence of each of the plurality of oligonucleotides is the same on the same particle but different on the different particle, and wherein at least 100 of the plurality of oligonucleotides associated with each of the plurality of particles comprise different molecular label sequences.
Dependencies are inferred from the claim text.