Heterotrophic Production Methods for Microbial Biomass and Bioproducts

An animal feed material or human food comprising non-encysted Chlamydomonadales microalgal cells or a purified product from said microalgal cells and produced according to a method, the method comprising:

• providing an organic acid as a carbon source into a culture medium;
• providing the microalgal cell that produces the feed material wherein the microalgal cell is a facultative heterotroph and is classified as part of the order Chlamydomonadales;
• culturing the microalgal cell in the culture medium in the dark to produce from the microalgal cell a microalgal culture comprising microalgal cells;
• isolating the microalgal cells from the microalgal culture after the cells reach a density of at least 6 g/L and before the cells form cyst cells;
• and
• purifying the animal feed material or human food from the isolated microalgal cells or obtaining a microalgal biomass comprising the microalgal cells as the animal feed material or human food;
• whereby the animal feed material or human food comprising the non-encysted Chlamydomonadales microalgal cells or the purified product from said microalgal cells is obtained.