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US 2021/0198628 A1Published application
Basal media for growing nk-92 cells
AssigneeNantKwest, Inc.
InventorsRichard John ANDERSON, John Charles GOULDING
Life of this patent
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Abstract
Provided herein are methods and customized media compositions for culturing NK-92® cells.
Claims33
A method of culturing NK-92® cells comprising culturing NK-92® cells in a customized NK-92® culture medium comprising a basal medium and one or more supplements,
- wherein the one or more supplements comprise ethanolamine, an ethanolamine derivative, insulin, transferrin, sodium selenite, HA, or a combination thereof;
- and
- wherein the basal medium comprises inorganic salts, vitamins and amino acids.
A cell culture comprising NK-92® cells in a customized NK-92® culture medium comprising a basal medium and one or more supplements,
- wherein the one or more supplements comprise ethanolamine, an ethanolamine derivative, insulin, transferrin, sodium selenite, HA, or a combination thereof;
- and
- wherein the basal medium comprises inorganic salts, vitamins and amino acids.
A cell culture comprising NK-92® cells in a customized NK-92® culture medium comprising a basal medium and one or more supplements,
- wherein the one or more supplements comprise ethanolamine, an ethanolamine derivative, or a combination thereof;
- and
- wherein the basal medium comprises inorganic salts, vitamins and amino acids.
Dependencies are inferred from the claim text.