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US 2021/0197202 A1Published application
Microfluidic siphoning array for nucleic acid quantification
AssigneeCombinati Incorporated
InventorsAndrew Zayac, Sammy Datwani, Megan Dueck, Ju-Sung Hung
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Abstract
The present disclose provides devices, methods and systems that may be used for amplifying and quantifying nucleic acid molecules. Methods for amplifying and quantifying nucleic acids may comprise isolating a sample comprising nucleic acid molecules into a plurality of chambers, performing a polymerase chain reaction on the plurality of chambers, and analyzing the results of the polymerase chain reaction.
Claims20
-52. (canceled)
A method for thermal cycling a microfluidic device, comprising:
- (a) providing a microfluidic device that is in fluid communication with a pneumatic module and that is in thermal communication with a thermal module, wherein said microfluidic device comprises a plurality of chambers, wherein said microfluidic device comprises a film or barrier that seals said plurality of chambers, wherein said film or barrier has a thermal conductivity of less than or equal to about 1 watt per meter Kelvin (W/m-K) at 20° C., and wherein said film or barrier is in thermal communication with said thermal module;
- (b) using said pneumatic module to load a nucleic acid sample comprising at least one nucleic acid molecule into a chamber of said plurality of chambers of said microfluidic device;
- (c) using said pneumatic module to apply a pressure to said microfluidic device to maintain thermal contact between said film or barrier of said microfluidic device and said thermal module;
- and
- (d) using said thermal module to thermal cycle said plurality of chambers to amplify said at least one nucleic acid molecule in said chamber, wherein a single round of thermal cycling is completed within about 20 seconds or less.
2 more elements
(canceled)
-78. (canceled)
Dependencies are inferred from the claim text.