Porous particle composite for pcr with heat dissipation function
AssigneeKorea Institute of Science and Technology
InventorsSang Kyung KIM, Bong Kyun KIM, Jung Min KIM
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Abstract
The present invention relates to a porous particle composite for PCR, wherein the porous particle composite distributes photothermal nano-elements that generate heat by absorbing light in porous particles in which nucleic acid amplification occurs through temperature control so as not to adjust the temperature of the entire sample by using a hot plate or the like but to adjust the temperature inside the particles by irradiating light to the porous particles to allow nucleic acid amplification inside thereof, thereby reducing energy consumption and shortening diagnostic time.
Claims14
A porous particle composite for PCR, comprising porous particles in which nucleic acid amplification occurs through temperature control and photothermal nanoelements that generate heat by absorbing light in the porous particles, wherein the temperature inside the porous particles can be adjusted to the temperature necessary for nucleic acid amplification by irradiating specific light to the porous particles.
A method for preparing a porous particle composite for PCR, comprising:
- a binding step of, by mixing one primer of a forward primer and a reverse primer in a solution wherein photothermal nanoelements are dispersed, binding the one primer onto the surface of the photothermal nanoelements;
- a first mixing step of forming a first mixture solution by mixing the photothermal nanoelements with the one primer bound and a porous particle-forming polymer solution;
- a second mixing step of forming a second mixture solution by mixing the first mixture solution with the other primer;
- and a curing step of preparing a porous particle composite having porosity, having the other primer of target nucleic acids fixed to inner pores and having the photothermal nanoelements with the one primer attached fixed to the inner pores by curing the second mixture solution.
Dependencies are inferred from the claim text.