Microfluidic device channel layer

A microfluidic system, comprising:

• a digital microfluidic electrode array (DMFEA), comprising at one least one die comprising a number of electrodes disposed along a surface of the die;
• a channel layer comprising:
• a number of sample wells located on a first side of the die;
• a number of first capillary channels fluidically coupled to each of the sample wells, the first capillary channels drawing a fluid from the sample wells using capillary forces;
• a capillary break fluidically coupled to each of the first capillary channels to dispense a portion of the fluid drawn from the sample wells through the capillary forces;
• a number of intermediate chambers fluidically coupled to the capillary break;
• a number of second capillary channels fluidically coupled to the intermediate chambers, the second capillary channels drawing the fluid from the intermediate chambers using capillary forces;
• and
• a number of mixing chambers fluidically coupled to the second capillary channels into which the capillary forces of the second capillary channels cause the fluid to enter the mixing chambers,
• wherein the electrodes cause the fluid to move out of the first capillary channels through the capillary break, through the intermediate chambers, and into the second capillary channels;
• and
• a printed circuit assembly (PCA) electrically coupled to the electrodes, the PCA controlling the activation of the electrodes.

A channel layer of a digital microfluidic device, comprising:

• a number of sample wells located on a first side of the die;
• a number of first capillary channels fluidically coupled to each of the sample wells, the first capillary channels drawing a fluid from the sample wells using capillary forces;
• a capillary break fluidically coupled to each of the first capillary channels to dispense a portion of the fluid drawn from the sample wells through the capillary forces;
• a number of intermediate chambers fluidically coupled to the capillary break;
• a number of second capillary channels fluidically coupled to the intermediate chambers, the second capillary channels drawing the fluid from the intermediate chambers using capillary forces;
• and
• a number of mixing chambers fluidically coupled to the second capillary channels into which the capillary forces of the second capillary channels cause the fluid to enter the mixing chambers.