Extracellular vesicle separation method, colloidal particle and preparation method thereof

A preparation method of a colloidal particle, the colloidal particle being used for extracellular vesicle separation, and the preparation method comprising:

• preparing an agarose solution with a concentration of 2 wt % to 6 wt % with water and agarose and heating the agarose solution to 90° C. to 100° C., and adding a surfactant with a concentration of 5 wt % to 20 wt % and a mineral oil with a concentration of 50 wt % to 60 wt % into the agarose solution for emulsification to form a first colloidal material;
• sieving the first colloidal material with a sieve to obtain a second colloidal material with a particle size ranging from 25 μm to 500 μm, and washing the second colloidal material with a detergent to remove oil stains and impurities;
• adding a cross-linking agent with a concentration of 0.1 M to 0.5 M into the washed second colloidal material to form a third colloidal material through stirring;
• and
• washing the third colloidal material with an organic solvent, and then surface-modifying the third colloidal material with a modification solution to obtain a colloidal particle surface-modified with biocompatible molecules,
• wherein the modification solution comprises sodium carboxymethyl cellulose (CMC), methyl cellulose (MC), glycine, aspartic acid, glutamic acid, bovine serum albumin (BSA), fetal bovine serum (FBS), or a combination thereof.