Methods of quantifying lif and uses thereof
AssigneesMedImmune, LLC, Fundacio Privada Institut d'Investigacio Oncologica de Vall Hebron, Fundació Privada Institució Catalana de Recerca | Estudis Avançats
InventorsJoan SEOANE SUAREZ, Judit ANIDO FOLGUEIRA, Peter Edward BAYLISS, Patricia Anne GIBLIN, Johan FRANSSON, Arif JETHA
Life of this patent
Estimating the term…
Abstract
Described herein are assays useful for the quantitation of total LIF from biological samples.
Claims25
A Leukemia Inhibitory Factor (LIF) complex, the complex comprising:
- LIF, a LIF capture antibody that specifically binds to LIF, a LIF detecting antibody that specifically binds to LIF, and optionally a LIF therapeutic antibody that specifically binds LIF, wherein the LIF detecting or the LIF capture antibody comprises A4 or a LIF binding fragment thereof .
A method of quantifying Leukemia Inhibitory Factor (LIF) in a sample from an individual comprising LIF comprising:
- a) contacting the sample comprising LIF to a capture antibody that specifically binds to LIF;
- b) contacting the sample comprising LIF to a detecting antibody that specifically binds LIF;
- c) detecting the LIF in the sample that is bound to the capture antibody and the detecting antibody;
- wherein the LIF detecting or the LIF capture antibody comprises A4 or a LIF binding fragment thereof .
A Leukemia Inhibitory Factor (LIF) binding antibody or fragment thereof, wherein the LIF binding antibody or fragment thereof comprises:
- a) an immunoglobulin heavy chain variable region with an amino acid sequence at least about 90% identical to the amino acid sequence set forth in SEQ ID NO:
- 41;
- and
- b) an immunoglobulin light chain variable region with an amino acid sequence at least about 90% identical to the amino acid sequence set forth in SEQ ID NO:
- 42.
Dependencies are inferred from the claim text.