New tagatose-6-phosphate 4-epimerase and application thereof
AssigneeTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
InventorsYanhe MA, Yuanxia SUN, Yunjie LI, Jiangang YANG
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Abstract
Disclosed is a new tagatose 6-phosphate 4-epimerase, which is capable of converting fructose 6-phosphate into tagatose 6-phosphate and vice versa. Also disclosed is an application of the enzyme in tagatose production.
Claims11
1-5. (canceled)
A method of using a tagatose 6-phosphate 4-epimerase (TiT4E) to catalyze mutual conversion between fructose 6-phosphate and tagatose 6-phosphate, wherein the tagatose 6-phosphate 4-epimerase is selected from the group consisting of:
- (a) a protein having the amino acid sequence set forth in SEQ ID NO:
- 1;
- or
- (b) a derived protein formed by substitution, deletion or addition of one or more amino acid residues in the protein having the amino acid sequence set forth in SEQ ID NO:
- 1, and having an activity of catalyzing mutual conversion between fructose 6-phosphate and tagatose 6-phosphate;
- or
- (c) a protein having a sequence comprising the sequence of the protein described in (a) or (b);
- or
- (d) a protein having an amino acid sequence of ≥60% (preferably ≥80%, more preferably ≥90%) identity to the amino acid sequence set forth in SEQ ID NO:
- 1, and having an activity of catalyzing mutual conversion between fructose 6-phosphate and tagatose 6-phosphate.
A method of using a tagatose 6-phosphate 4-epimerase (TiT4E) to construct an in vitro multi-enzyme catalytic system to prepare tagatose, wherein the tagatose 6-phosphate 4-epimerase is selected from the group consisting of:
- (a) a protein having the amino acid sequence set forth in SEQ ID NO:
- 1;
- or
- (b) a derived protein formed by substitution, deletion or addition of one or more amino acid residues in the protein having the amino acid sequence set forth in SEQ ID NO:
- 1, and having an activity of catalyzing mutual conversion between fructose 6-phosphate and tagatose 6-phosphate;
- or
- (c) a protein having a sequence comprising the sequence of the protein described in (a) or (b);
- or
- (d) a protein having an amino acid sequence of ≥60% (preferably ≥80%, more preferably ≥90%) identity to the amino acid sequence set forth in SEQ ID NO:
- 1, and having an activity of catalyzing mutual conversion between fructose 6-phosphate and tagatose 6-phosphate.
A method of using a host cell to construct an in vitro multi-enzyme catalytic system to prepare tagatose, wherein the host cell comprises a vector comprising a polynucleotide or has a polynucleotide integrated into its genome, and the polynucleotide is selected from the group consisting of:
- (a) a nucleotide sequence encoding the protein set forth in SEQ ID NO:
- 1;
- or
- (b) a nucleotide sequence set forth in SEQ ID NO:
- 2;
- or
- (c) a nucleotide sequence of ≥50% (preferably ≥80%, more preferably ≥90%) identity to the nucleotide sequence set forth in SEQ ID NO:
- 2;
- or
- (d) a nucleotide sequence which is complementary (preferably completely complementary) to the nucleotide sequence of any one of (a) to (c).
Dependencies are inferred from the claim text.