Back to results
US 2021/0171920 A1Published application
Modification of 3' Terminal Ends of Nucleic Acids by DNA Polymerase Theta
AssigneeTemple University of the Commonwealth System of Higher Education
InventorsRichard T. Pomerantz, Tatiana Kent
Life of this patent
Estimating the term…
Abstract
The invention provides compositions and methods for modifying the 3′-terminal ends of nucleic acids using DNA polymerase θ terminal transferase activity.
Claims30
A method of modifying a 3′ terminal end of a nucleic acid with a substrate, the method comprising:
- forming a mixture comprising an A family polymerase, a substrate, a nucleic acid, and a reaction solution, wherein the reaction solution comprises at least one divalent metal;
- incubating the mixture;
- and
- isolating a 3′-terminal end modified nucleic acid.
1 more element
A kit for modifying a 3′ terminal end of a nucleic acid with a substrate, the kit comprising an A-family polymerase and a reaction solution.
A method de novo synthesis of nucleic acids, the method comprising:
- forming a mixture comprising an A family polymerase, at least one nucleobase, and a reaction solution, wherein the reaction solution comprises at least one divalent metal;
- incubating the mixture;
- and
- isolating a nucleic acid.
1 more element
Dependencies are inferred from the claim text.