Functionalized surfaces and preparation thereof
AssigneeTwist Bioscience Corporation
InventorsBill James PECK, Pierre INDERMUHLE, Eugene P. MARSH, Andres FERNANDEZ, David STERN
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Abstract
Compositions, devices, methods and systems are provided for differential functionalization of a surface of a structure to support biopolymer synthesis. Provided herein are processes which include use of lamps, lasers, and/or microcontact printing to add functional groups to surfaces for the efficient and uniform synthesis of oligonucleic acids.
Claims44
A method for surface patterning, the method comprising:
- applying a first set of molecules to a surface of a structure, wherein each of the first set of molecules binds to the surface and comprises a reactive group capable of binding to a nucleoside;
- synthesizing a first layer of oligonucleotides, wherein each oligonucleotide in the first layer of oligonucleotides comprises about 10 to about 100 bases in length and extends from the surface;
- applying electromagnetic radiation (EMR) to a predetermined region of the surface to selectively remove a portion of the layer of oligonucleotides, wherein the EMR comprises a wavelength from about 100 nm to about 300 nm;
- and
- synthesizing a second layer of oligonucleotides, wherein each oligonucleotide in the second layer of oligonucleotides extends oligonucleotides from the remaining portion of the first layer of oligonucleotides.
A library of synthesized oligonucleic acids, comprising a plurality of different oligonucleic acids, each different oligonucleic acid extending from a structure at a different loci, wherein the different loci are at least about 75% uniform when measured by calculating amplitude of signal variation for oligonucleic acids extending from each locus divided by total signal intensity following white light illumination using an optical microscope.
A method for gene synthesis, the method comprising:
- providing predetermined sequences for a plurality of oligonucleic acids, wherein the plurality of oligonucleic acids collectively encode for a plurality of genes;
- providing a surface for oligonucleic acid synthesis;
- synthesizing the plurality of oligonucleic acids from the surface, wherein each oligonucleic acid extends from a different locus, and wherein the different loci are at least about 75% uniform when measured by calculating amplitude of signal variation for oligonucleic acids extending from each locus divided by total signal intensity following white light illumination using an optical microscope;
- and
- assembling the plurality of genes from the plurality of oligonucleic acids.
Dependencies are inferred from the claim text.