On demand, portable, cell-free molecular sensing platform
AssigneeNorthwestern University
InventorsMichael Christopher Jewett, Julius B. Lucks, Adam D. Silverman, Khalid K. Alam
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Abstract
Disclosed are methods, devices, kits, components, and compositions for detecting a target molecule in a test sample using a cell-free protein synthesis (CFPS) reaction. The methods, devices, kits, components, and compositions may be utilized for detecting target molecules which may include small molecules and/or metabolites of small molecules. The components and compositions used in the disclosed methods, devices, and kits may be dried or lyophilized and may be present or immobilized on a paper substrate, for example, as a paper test article.
Claims35
A method of detecting a target molecule in a biological or environmental sample, the method comprising:
- (i) obtaining a biological or environmental sample which may or may not contain the target molecule and optionally concentrating and/or solubilizing the target molecule in the sample if necessary;
- (ii) adding the sample and/or the optionally concentrated and/or solubilized target molecule in the sample to a cell-free protein synthesis (CFPS) reaction, wherein if the target molecule is present in the sample then an output is generated (e.g., a visual, electronic, or optical output).
Dependencies are inferred from the claim text.