Methods and compositions for detecting beta-hydroxybutyrate in biological fluids
AssigneeF.A.T. Stats LLC
InventorsTom Meitz, Frank Puglisi, Joseph Rokowski
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Abstract
Compositions and methods for detecting β-hydroxybutyrate in a biological fluid from a subject in need thereof are disclosed. Also disclosed are compositions and methods of reducing an optical change in a composition for detecting the β-hydroxybutyrate during storage before the composition is exposed to the β-hydroxybutyrate.
Claims20
A composition for detecting β-hydroxybutyrate (BHB), wherein the composition has an optical property that changes upon exposure to BHB, the composition comprising:
- (a) nitro tetrazolium blue (NZT) or 2,3,5-triphenyltetrazolium chloride,
- (b) β-hydroxybutyrate dehydrogenase (BHBD),
- (c) diaphorase, and
- (d) a redox cofactor, wherein the redox cofactor is 2,3-dimethoxy-5-methyl-p-benzoquinone (coenzyme Q0).
A composition for detecting β-hydroxybutyrate (BHB), wherein the composition has an optical property that changes upon exposure to BHB, comprising:
- (a) nitro tetrazolium blue (NZT) or 2,3,5-triphenyltetrazolium chloride,
- (b) β-hydroxybutyrate dehydrogenase (BHBD),
- (c) diaphorase,
- (d) a redox cofactor, wherein the redox cofactor is 2,3-dimethoxy-5-methyl-p-benzoquinone (coenzyme Q0) or nicotinamide dinucleotide (NAD), and
- (e) an inhibitor in an amount effective for reducing a change in the optical property of the composition for at least 6 hours before the composition is exposed to the BHB.
A method of preparing a composition for detecting β-hydroxybutyrate (BHB), comprising:
- (a) mixing nitro tetrazolium blue (NZT) or 2,3,5-triphenyltetrazolium chloride;
- β-hydroxybutyrate dehydrogenase (BHBD);
- diaphorase;
- and a redox cofactor to make a mixture, wherein the redox cofactor is 2,3-dimethoxy-5-methyl-p-benzoquinone (coenzyme Q0) or nicotinamide dinucleotide (NAD), and
- (b) adding to the mixture an inhibitor to make a composition having an optical property that changes upon exposure to BHB, wherein a change in the optical property of the composition is reduced for at least 6 hours before the composition is exposed to the BHB.
Dependencies are inferred from the claim text.