Methods and organisms with increased carbon flux efficiencies
AssigneeGenomatica, Inc.
InventorsAnthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Priti Pharkya, Tae Hoon Yang, Jungik Choi
Life of this patent
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Abstract
The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.
Claims12
A non-naturally occurring microbial organism comprising a genetic alteration selected from the group consisting of:
- (1) attenuation of cydA or cydB, and one or more of a genetic alteration selected from the group consisting of:
- (a) a genetic alteration that increases expression of a protein encoded by pntAB;
- (b) attenuation of the protein encoded by pykF;
- (c) attenuation of the protein encoded by sucCD;
- (d) attenuation of the protein encoded by yciA;
- (e) a genetic alteration that increases expression of a protein encoded by ackA and a protein encoded by pta;
- (f) a genetic alteration that increases expression of a protein encoded by cyoB;
- (g) attenuation of the protein encoded by pykA;
- (h) attenuation of the protein encoded by arcA;
- (i) attenuation of the protein encoded by crr;
- (j) attenuation of the protein encoded by clpA;
- and
- (k) attenuation of the protein encoded by menC;
- and
- (2) a genetic alteration selected from the group consisting of
- (a) attenuation of the proteins encoded by sucCD and yciA;
- (b) attenuation of the proteins encoded by sucCD and yciA, and having a genetic alteration that increases expression of a protein encoded by pntAB;
- (c) attenuation of the proteins encoded by sucCD and yciA, and having a genetic alteration that increases expression of a protein encoded by cyoB;
- (d) attenuation of the proteins encoded by sucCD, yciA and cydA or cydB, and having a genetic alteration that increases expression of a protein encoded by cyoB;
- (e) attenuation of the proteins encoded by sucCD, yciA and menC and having a genetic alteration that increases expression of a protein encoded by cyoB;
- (f) attenuation of the proteins encoded by sucCD, yciA, cydA or cydB and menC and having a genetic alteration that increases expression of an protein encoded by cyoB;
- (g) attenuation of the proteins encoded by sucCD and cydA or cydB;
- (h) attenuation of the proteins encoded by sucCD and cydA or cydB and pykF;
- (i) attenuation of the proteins encoded by sucCD and cydA or cydB, and having a genetic alteration that increases expression of a protein encoded by pntAB;
- (j) attenuation of the proteins encoded by sucCD and cydA or cydB and pykF, and having a genetic alteration that increases expression of a protein encoded by pntAB;
- (k) attenuation of the proteins encoded by sucCD, yciA and cydA or cydB;
- (l) attenuation of the proteins encoded by sucCD, yciA and cydA or cydB and pykF;
- (m) attenuation of the proteins encoded by sucCD, yciA and cydA or cydB, and having a genetic alteration that increases expression of a protein encoded by pntAB;
- (n) attenuation of the proteins encoded by sucCD, yciA and cydA or cydB and pykF, and having a genetic alteration that increases expression of a protein encoded by pntAB;
- (o) attenuation of the proteins encoded by sucCD, yciA, and cydA or cydB and menC;
- (p) attenuation of the proteins encoded by sucCD, yciA and cydA or cydB and menC, and having a genetic alteration that increases expression of a protein encoded by pntAB;
- (q) attenuation of the proteins encoded by sucCD and pykF, and having a genetic alteration that increases expression of a protein encoded by pntAB;
- (r) attenuation of the protein encoded by clpA;
- (s) attenuation of the protein encoded by menC;
- (t) attenuation of the protein encoded by menC and cydA or cydB;
- (u) attenuation of the protein encoded by pykF and/or pykA;
- (v) having a genetic alteration that increases expression of a protein encoded by pntAB;
- (w) attenuation of cydA or cydB and sucCD, arcA and crr, and having a genetic alteration that increases expression of a protein encoded by ackA and pta;
- (x) attenuation of cydA or cydB and arcA;
- (y) attenuation of cydA or cydB, and having a genetic alteration that increases expression of a protein encoded by pntAB;
- (z) attenuation of cydA or cydB and pykF;
- and
- (aa) attenuation of cydA or cydB and pykF, and having a genetic alteration that increases expression of a protein encoded by pntAB.
-7. (canceled)
A non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.
-40. (canceled)
A non-naturally occurring microbial organism comprising a gene disruption of a gene encoding YciA CoA hydrolase and a metabolically engineered pathway for producing a bioderived compound from a TCA cycle intermediate.
-70. (canceled)
-73. (canceled)
Dependencies are inferred from the claim text.