Monoclonal antibody targeting human tumor stem cells and use thereof

An isolated monoclonal antibody or antigen-binding fragment thereof against tumor stem cell, wherein the monoclonal antibody comprises a light chain variable region and a heavy chain variable region,

• the light chain variable region comprises:
• a VL CDR1 comprising an amino acid sequence set forth in SEQ ID NO:
• 2, or an amino acid sequence having 1 or 2 amino acid residue substitutions, deletions or additions relative to SEQ ID NO:
• 2,
• a VL CDR2 comprising an amino acid sequence set forth in SEQ ID NO:
• 3, or an amino acid sequence having 1 or 2 amino acid residue substitutions, deletions or additions relative to SEQ ID NO:
• 3, and
• a VL CDR3 comprising an amino acid sequence set forth in SEQ ID NO:
• 4, or an amino acid sequence having 1 or 2 amino acid residue substitutions, deletions or additions relative to SEQ ID NO:
• 4;
• the heavy chain variable region comprises:
• a VH CDR1 comprising an amino acid sequence set forth in SEQ ID NO:6, or an amino acid sequence having 1 or 2 amino acid residue substitutions, deletions or additions relative to SEQ ID NO:6,
• a VH CDR2 comprising an amino acid sequence set forth in SEQ ID NO:7, or an amino acid sequence having 1 or 2 amino acid residue substitutions, deletions or additions relative to SEQ ID NO:7, such as a sequence set forth in SEQ ID NO:13, and
• a VH CDR3 comprising an amino acid sequence set forth in SEQ ID NO:8, or an amino acid sequence having 1 or 2 amino acid residue substitutions, deletions or additions relative to SEQ ID NO:8, such as a sequence set forth in SEQ ID NO:14.