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US 2021/0147474 A1Published application
Methods of preparing samples for multiplex polypeptide sequencing
AssigneeQuantum-Si Incorporated
InventorsMatthew Dyer, Brian Reed
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Abstract
Aspects of the application provide methods of preparing a multiplexed sample for polypeptide sequencing, and compositions, kits and devices useful for the same.
Claims32
A method comprising:
- (i) contacting a population of polypeptides with a barcode component to produce a sample comprising one or more barcoded polypeptides;
- and
- (ii) combining the sample of (i) with one or more supplemental samples to generate a multiplexed sample for parallel polypeptide sequencing.
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A method comprising:
- (i) providing two or more populations of polypeptides;
- (ii) depositing the two or more populations of polypeptides of (i) on or within a solid substrate, wherein each population of polypeptides remains physically separated from the other populations of polypeptides in (i);
- thereby preparing a multiplexed sample for parallel polypeptide sequencing;
- optionally wherein at least one of the populations of polypeptides in (a):
- consists of a single polypeptide;
- comprises polypeptide fragments derived from a single polypeptide;
- or
- comprises a plurality of polypeptides.
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A device comprising a sample preparation module configured to interface with one or more cartridge, each cartridge comprising:
- (a) one or more reservoirs or reaction vessels configured to receive a complex sample;
- (b) one or more sequence sample preparation reagents, wherein the sample preparation reagents comprise a plurality of barcode molecules;
- and (c) a matrix comprising one or more immobilized capture probes, optionally wherein the sample preparation reagents further comprise a plurality of enrichment molecules.
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Dependencies are inferred from the claim text.