Modified sterol acyltransferases
AssigneeDsm IP Assets B.V.
InventorsChristopher Mark FARRELL, Lisa Ann LAPRADE, Otto Martin LEHMANN, Joshua TRUEHEART
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Abstract
The present invention is related to modified sterol acyltransferase enzymes with improved activity and/or specificity towards acylation of the vitamin D3 precursor 7-dehydrocholesterol (7-DHC) to be used in biotechnological production of vitamin D3. The invention further relates to a yeast strain expressing said modified enzymes and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.
Claims14
A modified enzyme having sterol acyltransferase activity comprising one of more amino acid substitution(s) at (a) position(s) corresponding to residues selected from 592 and/or 595 in the polypeptide according to SEQ ID NO:1, preferably substitution corresponding to F592L and/or G595D.
Dependencies are inferred from the claim text.