Kits suitable for use in electrochemiluminescence methods and methods of effecting a specific-binding non-wash assay
AssigneeMeso Scale Technologies, LLC.
InventorsMichael Tsionsky, Eli N. Glezer, Selen Stromgren, George Sigal, Jonathan K. Leland, Mark A. Billadeau, Svetlana Leytner, Mark Martin, Larry Helms
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Abstract
The invention relates to improved electrochemiluminescence assay methods for phosphorylated peptides or proteins employing phospho-specific antibodies and buffer compositions that are substantially free of inorganic phosphate.
Claims21
-28. (canceled)
A kit suitable for use in an electrochemiluminescence method, comprising (a) one or more containers configured to receive a sample, (b) a pH buffer comprising 75-150 mM ECL co-reactant, wherein said buffer has a pH of 7 to 8, has a concentration of less than 5 mM inorganic phosphate, and comprises a tertiary amine and 100-600 mM tris[hydroxymethyl]aminomethane (Tris).
Dependencies are inferred from the claim text.