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US 2021/0102230 A1Published application
Methods of manufacturing therapeutic proteins
AssigneesBiofactura, Inc., Momenta Pharmaceuticals, Inc.
InventorsRasheed TIJANI, Darryl Bacon SAMPEY, John ROBBLEE, Gan WEI, Chaomei HE
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Abstract
Disclosed herein are methods of manufacturing therapeutic proteins.
Claims25
-42. (canceled)
A method of manufacturing a therapeutic protein, comprising:
- transfecting a cholesterol-auxotrophic cell with (i) a nucleic acid encoding a protein that restores cholesterol biosynthesis in the cell;
- (ii) an aminoglycosidic antibiotic resistance gene;
- and (iii) a nucleic acid encoding the therapeutic protein.
A transformed mammalian host cell, transfected with an aminoglycosidic antibiotic resistance gene, and a nucleic acid encoding a therapeutic protein, wherein the transformed mammalian host cell is derived from a parental mammalian host cell that is cholesterol-auxotrophic.
Dependencies are inferred from the claim text.