Genome editing method, composition, cell, cell preparation, and method for producing cell preparation
AssigneeJichi Medical University
InventorsYutaka Hanazono, Hiromasa Hara, Hideki Uosaki
Life of this patent
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Abstract
A genome editing method for an isolated cell, the method comprising introducing foreign DNA into a targeted genome with homologous recombination of at least one of a 5′-end or a 3′-end of the foreign DNA, where the foreign DNA has homology arms, each with a length of less than 500 bp.
Claims20
A genome editing method which is a method for editing a genome in an isolated cell, the method comprising introducing foreign DNA into a targeted genome with homologous recombination of one of a 5′-end or a 3′-end of the foreign DNA and non-homologous recombination of the other end when double-strand breaks of a targeted genomic DNA occur, where the foreign DNA has homology arms, and a length of one homology arm is two times or more a length of the other homology arm.
A genome editing method which is a method for editing a genome in an isolated cell, the method comprising introducing foreign DNA into a targeted genome with homologous recombination of both of a 5′-end and a 3′-end of the foreign DNA when double-strand breaks of a targeted genomic DNA occur, where the foreign DNA has homology arms, each with a length of less than 500 bp, wherein the cell is a blood cell or a hematopoietic stem cell.
-13. (canceled)
A method for producing a cell preparation for treating severe combined immunodeficiency, the method comprising, in a cell, introducing foreign DNA into a genome of the cell with homologous recombination of one of a 5′-end or a 3′-end of the foreign DNA and non-homologous recombination of the other end when double-strand breaks of a targeted genomic DNA occur, where the foreign DNA has homology arms, each with a length of less than 500 bp, and at the 5′-end and the 3′-end, and a length of one homology arm is two times or more a length of the other homology arm.
A method for producing a cell preparation for treating severe combined immunodeficiency, the method comprising, in a cell, introducing foreign DNA into a genome of the cell with homologous recombination of both of a 5′-end and a 3′-end of the foreign DNA when double-strand breaks of a targeted genomic DNA occur, where the foreign DNA has homology arms, each with a length of less than 500 bp, the cell is a blood cell or a hematoppietic stem cell.
-26. (canceled)
Dependencies are inferred from the claim text.