Peptides that block transmission of orthotospoviruses and methods of using the same
AssigneeBoard of Trustees of the University of Arkansas
InventorsIoannis Tzanetakis, Jing Zhou
Life of this patent
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Abstract
Orthotospovirus virions travel through the thrips foregut and enter midgut epithelial cells through the interaction between virus glycoproteins and cellular receptors with several protein motifs thought to be involved in the interaction. Single, double and triple mutant polypeptides in the soybean vein necrosis virus (SVNV)/Neohydatothrips variabilis system are provided herein and several are shown to block viral transmission from the thrips to the soybean plants. Methods for inhibiting viral transmission using these polypeptides or constructs comprising polynucleotides encoding peptides are also provided herein.
Claims21
A composition comprising a polypeptide comprising or consisting of an amino acid sequence selected from the group consisting of:
- a.
- SEQ ID NO:
- 1
- (NASIRGDHEVSQE);
- b.
- SEQ ID NO:
- 2
- (RLTGECNITKVSLTN);
- c.
- SEQ ID NO:
- 3
- (NASIAAAHEVSQE);
- d.
- SEQ ID NO:
- 4
- (NASIAGDHEVSQE);
- e.
- SEQ ID NO:
- 5
- (NASIRADHEVSQE);
- f.
- SEQ ID NO:
- 6
- (NASIRGAHEVSQE);
- and a polypeptide having 90% sequence identity to one of SEQ ID NOs:
- 1-6 and combinations thereof.
The method of clam 14, wherein the viral transmission inhibited is from the larvae to the plants.
Dependencies are inferred from the claim text.