Methods to evaluate traits
AssigneePioneer Hi-Bred International, Inc.
InventorsNATHAN DAVID COLES, JEFFREY M HEGSTAD, JASDEEP S MUTTI
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Abstract
The present disclosure provides methods for testing agronomic performance of transgenic traits and genome edits in plants and for accelerated selection of such plants. Methods evaluated include testing constructs for transgenic trait performance (T) using isolines. The methods comprise crossing events into different genetic backgrounds. Methods also comprise combining isoline data with F2:3 bulk data and developing breeding values across different genetic backgrounds for a higher level of confidence in selecting events.
Claims34
A method for selecting a plant comprising an introduced genetic modification conferring a trait of interest, the method comprising:
- (a) obtaining a F2 population of segregating plants from a F1 population resulting from crossing a plant line that contains the genetic modification to a second plant line that does not contain the genetic modification;
- (b) generating a bulked pool of F2 population of plants that are homozygous positive for the genetic modification that is designated homozygous positive pool and generating a separate bulked pool of F2 plants that are homozygous negative for the genetic modification that is designated homozygous negative pool;
- (c) phenotyping or genotyping a plurality of plants from the homozygous positive pool and the homozygous negative pool;
- (d) assigning a breeding value to each plant or a subset of the plants in the bulked pool of plants that are homozygous positive for the genetic modification based on the phenotyping or the genotyping;
- and
- (e) selecting the plant comprising the genetic modification based on the breeding value.
A method for selecting a transgenic event or a genome edited plant, the method comprising:
- (a) obtaining F1 offspring from a cross resulting between a plant line that contains a transgene of interest or a genome modification to a second plant line that does not contain said transgene or said genome modification;
- (b) creating a double haploid (DH) plant population or a recombinant inbred line (RIL) plant population based on the F1 offspring;
- (c) conducting an assay to determine which offspring from said population contain the transgene or the genome modification;
- (d) selecting a pool of plants that are homozygous positive for the transgenic event or the genome modification and a separate pool of plants that are homozygous negative for the transgenic event or the genome modification;
- (e) phenotyping or genotyping the homozygous positive pool and the homozygous negative pool;
- (f) assigning a breeding value to each transgenic event or a subset of the transgenic events or each genome edited plant or a subset of the genome edited plants, in the bulked pool of plants that are homozygous positive for the transgenic event or the genome modification based on the phenotyping or genotyping;
- and
- (g) selecting the transgenic event based on the breeding value.
A method for accelerated selection of a transgenic event or a genome edited plant, the method comprising:
- (a) crossing a plant line that is homozygous positive for the transgenic event or a genome edited plant with a diverse panel of plant lines that do not contain said transgene;
- (b) crossing an isogenic transgene null of (a) with the same panel of plant lines of (a) that do not contain said transgene or genome modification;
- (c) collecting offspring of said crosses of (a) and (b) to produce transgene or genome modification positive and transgene or genome modification negative hybrids;
- (d) phenotyping or genotyping the hybrids that are positive for the transgene or genome modification and the hybrids that are negative for the transgene or genome modification;
- (e) assigning a breeding value to each transgenic event or genome edited plant or a subset of the transgenic events or genome modifications in the transgene positive hybrids or genome edited hybrids based on the phenotyping or the genotyping;
- and
- (f) selecting the transgenic event or genome modification based on the breeding value.
A method for accelerated selection of a genome edited plant, the method comprising:
- (a) crossing a genome edited plant line that comprises an introduced genome modification with a plant line that does not contain said genome modification;
- (b) collecting F1 offspring from said cross;
- (c) creating an early generation bi-parental bulk plant population;
- (d) conducting an assay to determine which of said offspring contain the genome modification of interest;
- (e) creating a bulked pool of plants that are homozygous positive for the genome modification and a different bulked pool of plants that are homozygous negative for the genome modification;
- (f) phenotyping or genotyping the homozygous positive pool and the homozygous negative pool;
- (g) assigning a breeding value to each genome edited plant in the bulked pool of plants that are homozygous positive for the genome modification based on the phenotyping or the genotyping;
- and
- (h) selecting the genome edit plant based on the breeding value.
34-40. (canceled)
Dependencies are inferred from the claim text.