Methods of increasing nutrient use efficiency
AssigneeInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences
InventorsXiangdong Fu, Shan Li, Kun Wu, Yonghang Tian, Qian Liu
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Abstract
The invention relates to methods for increasing nitrogen uptake, nitrogen assimilation, nitrogen use efficiency as well as yield in a plant, without affecting plant height, the method comprising increasing the expression or levels of a growth regulatory factor (GRF). Also described are genetically altered plants characterised by the above phenotype as well as methods of producing such plants.
Claims118
A method of increasing nitrogen uptake and/or nitrogen assimilation and/or nitrogen use efficiency in a plant, the method comprising increasing the expression or levels of a growth regulatory factor (GRF) or increasing the activity of a growth regulatory factor.
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A genetically altered plant, part thereof or plant cell, wherein the expression or level of a growth regulatory factor (GRF) or activity of a GRF is increased compared to a wild-type or control plant, and wherein the plant is characterised by an increase in at least one of nitrogen uptake and/or nitrogen assimilation and/or nitrogen use efficiency compared to a wild-type or control plant.
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A method for identifying and/or selecting a plant that will have increased nitrogen uptake and/or nitrogen assimilation and/or nitrogen use efficiency, preferably compared to a wild-type or control plant, the method comprising detecting in the plant or plant germplasm at least one polymorphism in at least one GRF gene or GRF promoter and selecting said plant or progeny thereof.
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A nucleic acid construct comprising a nucleic acid sequence encoding at least one DNA-binding domain that can bind to at least one GRF gene wherein the nucleic acid sequence encodes at least one protospacer element, and wherein the sequence of the protospacer element is selected from SEQ ID Nos 50, 53, 58, 61, 66, 69, 74, 77, 82, 86, 91, 94, 99, 102, 107, 110, 115, 118, 123, 126. 131, 134, 139, 142, 147, 150, 155, 158, 163, 166, 171, 174, 179, 182, 187 and 190 or a sequence that is at least 90% identical to SEQ ID Nos 50, 53, 58, 61, 66, 69, 74, 77, 82, 86, 91, 94, 99, 102, 107, 110, 115, 118, 123, 126. 131, 134, 139, 142, 147, 150, 155, 158, 163, 166, 171, 174, 179, 182, 187 and 190, or at least one a DNA donor nucleic acid selected from 48, 56, 64, 72, 80, 84, 89, 97, 105, 113, 121, 129, 137, 145, 153, 161, 169, 177 and 185 or a variant thereof.
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A single guide (sg) RNA molecule wherein said sgRNA comprises a crRNA sequence and a tracrRNA sequence, wherein the crRNA sequence can bind to at least one sequence selected from SEQ ID Nos 49, 52, 57, 60, 65, 68, 73, 76, 81, 85, 90, 93, 98, 101, 106, 109, 114, 117, 122, 125, 130, 133, 138, 141, 146, 149, 154, 157, 162, 165, 170, 173, 178, 181, 186 and 189 or a variant thereof.
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Dependencies are inferred from the claim text.