Gadusol derivative production in bacteria
AssigneeOregon State University
InventorsTaifo Mahmud, Andrew Robert Osborn
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Abstract
Disclosed is a synthetic gene cluster for producing gadusol derivatives, expression vectors and host cells containing the same, methods of producing gadusol derivatives, and compositions thereof. In an example, the synthetic gene cluster includes a valA nucleotide sequence capable of expressing ValA protein; a nucleotide sequence capable of expressing methyltransferase/oxidoreductase (MT-Ox) protein; a mysC nucleotide sequence capable of expressing a MysC protein; and a mysD nucleotide sequence capable of expressing a MysD protein. In this way, gadusol derivatives can be produced in amounts sufficient for use in a variety of applications.
Claims20
A synthetic gene cluster, comprising:
- a valA nucleotide sequence capable of expressing ValA;
- a nucleotide sequence capable of expressing methyltransferase/oxidoreductase (MT-Ox) protein;
- a mysC nucleotide sequence capable of expressing a MysC protein;
- and
- a mysD nucleotide sequence capable of expressing a MysD protein.
A bacterium with an expression vector with a synthetic gene cluster comprising:
- a valA nucleotide sequence capable of expressing ValA;
- a nucleotide sequence capable of expressing methyltransferase/oxidoreductase (MT-Ox) protein;
- a mysC nucleotide sequence capable of expressing a MysC protein;
- and
- a mysD nucleotide sequence capable of expressing a MysD protein.
A method of producing one or more gadusol derivatives, comprising, culturing a host cell having an expression vector with a synthetic gene cluster comprising:
- a valA nucleotide sequence capable of expressing ValA;
- a nucleotide sequence capable of expressing methyltransferase/oxidoreductase (MT-Ox) protein;
- a mysC nucleotide sequence capable of expressing a MysC protein, and a mysD nucleotide sequence capable of expressing a MysD protein;
- and
- isolating one or more gadusol derivatives from the culture.
A sunscreen compound having the structure:
Dependencies are inferred from the claim text.