Systems and Methods for Electronic Surface Antigen Expression Analysis Using Magnetophoresis

A microfluidic device comprising,

• a first inlet configured to receive a first fluid;
• a first flow chamber having a first outlet and a second outlet, the first outlet exiting to a second flow chamber, and the second outlet exiting to a removal channel;
• a first magnet disposed adjacent to the first flow chamber, the first magnet configured to separate magnetically-labeled cells of the first fluid from non-labeled cells of the first fluid by diverting the magnetically-labeled cells to the first outlet;
• a plurality of bins, each bin having a first end and a second end, the first end of each bin in fluidic communication with the second flow chamber and disposed distal from the first fluid inlet;
• a second magnet disposed adjacent to the second flow chamber, the second magnet configured to attract the magnetically-labeled cells of the first fluid towards a bin of the plurality of bins;
• and
• a plurality of sensors, each sensor disposed at the second end of a corresponding bin of the plurality of bins, each sensor configured to:
• produce a unique signal in response to detecting a cell of the magnetically-labeled cells of the first fluid passing through the bin corresponding to the sensor;
• and
• detect a magnetism of a cell of the plurality of magnetically-labeled cells;
• wherein each sensor is coded with a multi-bit Gold sequence to produce the unique signal.

A method for antigen expression analysis in whole blood, the method comprising:

• combining functionalized magnetic particles with blood, wherein the functionalized magnetic particles create a plurality of targeted cells and non-targeted cells, the targeted cells being magnetically-labeled;
• providing a microfluidic device comprising:
• a first inlet to receive the blood with targeted and non-targeted cells;
• a first flow chamber having a first fluid outlet and a second fluid outlet, the first fluid outlet exiting to a second flow chamber, and the second fluid outlet exiting to a removal channel;
• a first magnet disposed adjacent to the first flow chamber, the first magnet configured to separate the targeted and non-targeted cells by (i) diverting the targeted cells to the first fluid outlet and (ii) allowing the non-targeted cells to flow to the second fluid outlet and to the removal channel;
• a plurality of bins, each bin having a first end and a second end, the first end of each bin in fluid communication with the second flow chamber and disposed distal to the first fluid inlet;
• a second magnet disposed adjacent to the second flow chamber, the second magnet configured to attract the targeted cells towards a bin of the plurality of bins;
• and
• a plurality of sensors, each sensor disposed at the second end of a corresponding bin of the plurality of bins, each sensor configured to produce a unique signal in response to detecting a targeted cell;
• delivering the blood and cells into the first inlet;
• flowing the blood from the first inlet and through the first flow chamber to separate the targeted cells from the non-targeted cells;
• flowing the blood from the first fluid outlet, through the second flow chamber, and through the plurality of bins;
• and
• receiving the unique signal from a sensor of the plurality of sensors.