Mass spectrometry assay for estrogenic compounds
AssigneeQuest Diagnostics Investments Incorporated
InventorsMildred GOLDMAN, Nigel J. CLARKE
Life of this patent
Estimating the term…
Abstract
Methods are provided for detecting the amount of one or more HRT panel analytes (i.e., estrone (E1), estrone sulfate (E1s), 17α-estradiol (E2a), 17β-estradiol (E2b), estradiol sulfate (E2s), estriol (E3), equilin (EQ), 17α-dihydroequilin (EQa), 17β-dihydroequilin (EQb), Equilenin (EN), 17α-dihydroequilenin (ENa), 17β-dihydroequilenin (ENb), and Δ8,9-dehydroestrone (dE1)) in a sample by mass spectrometry. The methods generally involve ionizing one or more HRT panel analytes in a sample and quantifying the generated ions to determine the amount of one or more HRT panel analytes in the sample. In methods where amounts of multiple HRT panel analytes are detected, the amounts of multiple analytes are detected in the same sample injection.
Claims8
A method for determining the amount of 17β-estradiol and estrone by mass spectrometry, the method comprising:
- a. adding one or more deuterated internal standards of 17β-estradiol and/or estrone to a sample containing 17β-estradiol and estrone;
- b. purifying the sample by high performance liquid chromatography (HPLC);
- c. ionizing the sample under conditions suitable to produce one or more ions of 17β-estradiol and estrone detectable by mass spectrometry;
- d. determining the amounts of one or more ions of 17β-estradiol and estrone by tandem mass spectrometry;
- and
- e. using the amounts of one or more ions from each of 17β-estradiol and estrone to determine the amounts of each of 17β-estradiol and estrone in the sample of the individual.
The method of claim 5, wherein further comprising turbulent flow liquid chromatography (TFLC) prior to HPLC.
The method of claim 10, wherein the internal standard is d4-estrone (E1-d4) and d5-17β-estradiol (E2b-d5).
Dependencies are inferred from the claim text.