Methods for rapidly digesting biopolymers with ultrastable enzymes for mass spectrometry-based analyses
AssigneeCinder Biological, Inc.
InventorsSteven M. YANNONE, Jill O. FUSS, Adam BARNEBEY
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Abstract
The present disclosure relates to a composition containing an ultrastable enzyme, methods of using the same for preparing a biological sample for analysis by mass spectrometry, and kits comprising the same. The composition includes an ultrastable enzyme isolated from a hyperthermophilic and/or acidophilic organism and optionally, an acid and an additive. The composition can be used at temperatures ranging from about 50° C. to 110° C., preferably at temperatures ranging from about 70° C. to 100° C. In addition, the composition can be used at pH values ranging from 0.5 to 7, preferably at pH values ranging from 2 to 5.
Claims74
A method of preparing a biological sample, comprising:
- (a) providing the biological sample comprising at least one biopolymer;
- (b) contacting the sample with a composition comprising an ultrastable enzyme to form a reaction mixture;
- and
- (c) incubating the reaction mixture for at least one second to digest or modify the at least one biopolymer present in the biological sample,
- wherein steps (a) to (c) produce a prepared sample suitable for proteomic, glycomic, glycoproteomic analysis.
Dependencies are inferred from the claim text.