Materials and methods for performing histochemical assays for human pro-epiregulin and amphiregulin
AssigneeVentana Medical Systems, Inc.
InventorsFernando Jose Rebelo do Couto, Zhiming Liao, Andrea Muranyi, Kandavel Shanmugam, Shalini Singh, Yifei Zhu
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Abstract
The invention provides anti-human pro-epiregulin and anti-human amphiregulin antibodies and methods of using the same. Anti-EREG antibodies raised against amino acids 148-169 and 156-169 of the human EREG protein, and anti-AREG antibodies raised against amino acids 238-252 of the human AREG protein are disclosed. Methods of using these antibodies to detect EREG and AREG and kits and other products for performing such methods are also disclosed.
Claims34
An antibody or antibody fragment selected from the group consisting of:
- an antibody or antibody fragment capable of specifically binding to amino acids 148-169 of SEQ ID NO:
- 1;
- and
- an antibody or antibody fragment capable of specifically binding to amino acids 156-169 of SEQ ID NO:
- 1.
An antibody or antibody fragment selected from the group consisting of:
- (a) an antibody or antibody fragment comprising a VH sequence of SEQ ID NO:
- 16 and a VL sequence of SEQ ID NO:
- 17;
- (b) an antibody or antibody fragment comprising a VH sequence of SEQ ID NO:
- 33, and a VL sequence of SEQ ID NO:
- 34 or SEQ ID NO:
- 35;
- (c) an antibody or antibody fragment specific for SEQ ID NO:
- 1 that competes for binding to SEQ ID NO:
- 1 with an antibody comprising the VH sequence of SEQ ID NO:
- 16 and the VL sequence of SEQ ID NO:
- 17;
- and
- (d) an antibody or antibody fragment specific for SEQ ID NO:
- 1 that competes for binding to SEQ ID NO:
- 1 with an antibody comprising the VH sequence of SEQ ID NO:
- 33, and the VL sequence of SEQ ID NO:
- 34 or SEQ ID NO:
- 35.
Dependencies are inferred from the claim text.