Creatine kinase isoenzyme assay kit

A kit for detecting creatine kinase isoenzyme by latex-enhanced immunoturbidimetry, comprising a first reagent and a second reagent,

• wherein the first reagent comprises:
• 10 mM-100 mM
• buffer,
• 5 g/L-50 g/L
• electrolyte,
• 1 g/L-10 g/L
• polyethylene glycol,
• 1 g/L-20 g/L
• surfactant,
• 0.5 g/L-1.5 g/L
• preservative,
• 0.1%-2%, v/v
• blocking agent,
• 1 g/L-20 g/L
• protective agent,
• pH 6.5-8.51;
• the second reagent comprises:
• 10 mM-100 mM
• buffer,
• 0.1%-0.5%, w/v
• polystyrene latex particle coated with
• antibody against creatine kinase isoenzyme,
• 0.02 g/L-0.05 g/L
• antibody against creatine kinase isoenzyme
• on said latex particle,
• 1 g/L-20 g/L
• protective agent,
• 20 g/L-200 g/L
• stabilizer,
• 0.5 g/L-1.5 g/L
• preservative,
• pH 6.5-8.51;
• the buffer is selected from the group consisting of glycine buffer, TRIS-HCl buffer and HEPES buffer;
• the preservative is selected from the group consisting of sodium azide, thimerosal and ProClin300;
• the polyethylene glycol is selected from the group consisting of PEG 6000, PEG 8000, PEG 12000 and PEG 20000;
• the surfactant is selected from the group consisting of Tween 20, Tween 80, NP40 and thesit;
• the protective agent is selected from the group consisting of bovine serum albumin, ovalbumin, skim milk and calf serum;
• the blocking agent is goat anti-mouse IgG;
• the stabilizer is selected from the group consisting of sucrose, glycerol, trehalose and glucose;
• the surface functional group of the polystyrene latex particle is selected from the group consisting of amino group, carboxyl group, chloromethyl group and epoxy group;
• the polystyrene latex particle has an average particle size of from 200 nm to 500 nm;
• the antibody against creatine kinase isozyme is derived from mouse or goat;
• and
• the antibody against creatine kinase isozyme is monoclonal antibody.