Quantitative analyte detection in lateral flow immunochemistry
AssigneeMireya C. Aronowitz
InventorJACK L. ARONOWITZ
Life of this patent
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Abstract
Lateral flow immunochemistry testing systems and methods are provided. A system can include a tester substrate with at least two control lines and at least one test sample line. A test sample with an unknown amount of analyte can be deposited on the tester substrate and the test sample can move along the tester substrate, to contact the at least two control lines and the at least one test sample line. A measuring device can be used to compare the at least one test sample line to the at least two control lines to give a quantitative value of the amount of analyte present in the test sample.
Claims20
A tester substrate, configured to obtain a quantitative amount of an analyte in a test sample, the tester substrate comprising:
- at least one test sample zone comprising a moiety-binding substance that binds to a moiety that is the analyte or is bound to the analyte;
- and
- at least two control zones, each control zone comprising a known different amount of the moiety-binding substance, and configured such that when contacted with the analyte in the test sample, binds with a corresponding known amount of the moiety in the test sample, such that obtaining a measure of the amount of moiety bound to each control zone provides standards against which a measure obtained for the amount of moiety bound to the test sample zone can be compared to determine a quantitative amount of the analyte in the test sample.
A kit for obtaining a quantitative amount of an analyte in a test sample, the kit comprising:
- at least one tester substrate having:
- at least one test sample zone comprising an moiety-binding substance;
- and
- at least two control zones each comprising known different amounts of the moiety-binding substance,
- such that, when the analyte contacts the moiety-binding substance in the at least one test sample zone a test sample line is formed and, when the analyte contacts the moiety-binding substance in the at least two control zones and a control zone line is formed in each;
- and
- a measuring device, having access to a calibration curve, that measures each of the control zone lines and compares the measurements with the calibration curve to determine a high standard and a low standard on the calibration curve that correspond to the known amounts of moiety-binding substance present in each of the respective control zones and that measures the test sample line and compares the test sample line to the high standard and the low standard to determine the amount of moiety bound to the moiety-binding substance in the test sample zone.
Dependencies are inferred from the claim text.