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US 2021/0063384 A1Published application
Method of assessing circulating tumor cells and method of using data obtained therefrom
AssigneeChang Gung University
InventorsMIN-HSIEN WU, CHIA-JUNG LIAO
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Abstract
Methods of assessing circulating tumor cells (CTCs) and methods of using the data obtained from assessing CTCs are provided. The methods employ negative selection and 3D cell culture techniques to isolate and culture CTCs. Those CTCs then can be used in different bioassays or evaluations, such as to determine tumor morphology, monitor tumor status, predict prognosis, provide treatment suggestion, and assess treatment effectiveness.
Claims26
A method of assessing circulating tumor cells (CTCs), comprising:
- providing a specimen, wherein the specimen is peripheral blood and comprises multiple non-target cells and multiple target cells;
- removing a first subset of the multiple non-target cells;
- sustaining the multiple target cells;
- incubating the specimen with a fluorescent dye to label multiple CTCs in the multiple target cells;
- identifying cells having metastatic potential and cells having metastatic characteristics among the multiple CTCs;
- obtaining a biological data from the cells having metastatic potential and the cells having metastatic characteristics respectively;
- and
- applying a first bioassay, selected based on the biological data, to the cells having metastatic potential and the cells having metastatic characteristics.
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