Collagenase loaded liposomes for enhancing drug delivery
AssigneeTechnion Research & Development Foundation Limited
InventorsAvi SCHROEDER, Assaf ZINGER
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Abstract
Pharmaceutical compositions comprising at least one lipid-based particle encapsulating a proteolytic enzyme and a pharmaceutically acceptable carrier, wherein the proteolytic enzyme comprises at least 75% proteolytically active enzyme are provided. Methods of using same and producing same are also provided.
Claims30
A pharmaceutical composition comprising at least one lipid-based particle encapsulating at least one proteolytic enzyme and a pharmaceutically acceptable carrier, wherein at least 75% of said proteolytic enzyme is proteolytically active.
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A method of increasing penetrance of a drug into a tumor in a subject in need thereof, the method comprising:
- a. administering to the subject a pharmaceutical composition comprising at least one lipid-based particle encapsulating at least one proteolytic enzyme and a pharmaceutically acceptable carrier;
- and
- b. administering said drug to said subject,
- thereby increasing the penetrance of said drug into said tumor.
A method of producing a pharmaceutical composition comprising a liposome encapsulating at least one enzyme, the method comprising:
- a. providing a thin lipid film;
- and
- b. rehydrating said thin lipid film with a solution comprising said enzyme, wherein said rehydrating produces liposomes encapsulating said enzyme and wherein said solution comprising said enzyme
- i. is devoid of organic solvents;
- ii. is devoid of ions that activate said enzyme;
- and
- iii. comprises a temperature below a transition temperature of a lipid in said thin lipid film;
- thereby producing liposomes encapsulating at least one enzyme.
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Dependencies are inferred from the claim text.