Immunochromatographic test strip for detecting digoxin and uses thereof

An immunochromatographic test strip for detecting digoxin, comprising a bottom plate, and a sample pad, a glass fiber mat, a nitrocellulose membrane and a water absorbing paper which are sequentially overlapped on the bottom plate, wherein the glass fiber mat is sprayed with a digoxin-specific antibody-fluorescent microsphere complex;

• and the nitrocellulose membrane is sequentially provided with a detection line coated with digoxin protein conjugate and a quality control line coated with a secondary antibody;
• the digoxin-specific antibody-fluorescent microsphere complex is obtained by conjugation between a digoxin monoclonal antibody or a digoxin polyclonal antibody and a fluorescent microsphere;
• and
• the digoxin protein conjugate comprises a digoxin-conjugated rabbit albumin or a digoxin-conjugated rabbit ovalbumin;
• the mass percentage concentration of the digoxin-specific antibody-fluorescent microsphere complex is 0.01-0.1%;
• and the spraying amount of the digoxin-specific antibody-fluorescent microsphere complex on the glass fiber mat is 1-10 μl/cm;
• the test sample comprises serum, plasma and whole blood.