Regulation of csr system for production of lysine and lysine-derived products
AssigneesCathay Biotech Inc., Cibt America Inc.
InventorsHoward CHOU, Ling CHEN, Yunfeng LEI, Xiucai LIU
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Abstract
The invention provides microorganisms genetically modified to overexpress biofilm dispersal related polypeptides to enhance the production of lysine and lysine derivatives by the microorganism, method of generating such microorganism, and methods of producing lysine and lysine derivatives using the genetically modified microorganisms.
Claims30
A genetically modified host cell comprising an exogenous nucleic acid encoding a CsrB sRNA or a CsrC sRNA, wherein the host cell overexpresses the CsrB sRNA or CsrC sRNA relative to a counterpart host cell that has not been modified to express the exogenous nucleic acid;
- and has at least one additional genetic modification to increase production of lysine or a lysine derivative compared to a wildtype host cell.
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A method of engineering a host cell to increase production of lysine or a lysine derivative, the method comprising introducing an exogenous nucleic acid encoding a CsrB or CsrC sRNA into the host cell, wherein the host cell has at least one additional genetic modification to increase production of lysine or a lysine derivative compared to a wildtype host cell;
- culturing the host cell under conditions in which the CsrB or CsrC sRNA is expressed, and
- selecting a host cells that exhibits increased production of lysine or a lysine derivative relative to a counterpart control host cell that has not been modified to express the exogenous nucleic acid.
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Dependencies are inferred from the claim text.