Systems and methods for evaluating immune response to infection
AssigneeBeckman Coulter, Inc.
InventorsLiliana TEJIDOR, Robert T. MAGARI, Diana CAREAGA, Mohamad HASAN, Sanghyuk SHIN
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Abstract
Systems and methods for characterizing immune response to infection using cellular analysis, such as a hematological cellular analyzer. In some instances, the immune response may be characterized as normal or abnormal based on one or more blood cell population parameters. In some instances, abnormal characterization may be used to identify patients with sepsis or at elevated risk of developing sepsis.
Claims20
A method for characterizing inflammatory response to infection, the method comprising:
- a. flowing a body fluid sample through a flowcell, the body fluid sample comprising a heterogenous population of circulating cells;
- b. detecting whether a differentially expressed sepsis cell population parameter is present in the heterogenous population of circulating cells by analyzing one or more cell population parameters selected from the group consisting of MO_DC_SD, NE_DC_SD, NE_DC_MEAN, NNRBC_UMALS_SD, MO_ALL_SD, NE_NO, LY_PC, NNRBC_MALS_SD, WNOP, MO_DC_MEAN, WDOP, NNRBC_UMALS_MEAN, BA_PC, NNRBC_MALS_MEAN, BA_PC, NNRBC_MALS_MEAN, EGC_LMALS_MEAN, NNRBC_DC_SD, NNRBC_LMALS_SD, NNRBC_ALL_MEAN, and combinations thereof based on light scatter and/or direct current impedance measurements of the body fluid sample;
- and
- c. characterizing an inflammatory response to infection as abnormal based at least in part on detecting the differentially expressed sepsis cell population parameter.
A system for characterizing inflammatory response to infection, the system comprising:
- a. a transducer module configured to measure at least light scatter and direct current impedance caused by cells passing through a flowcell;
- and
- b. a processor configured with a set of instructions stored on a non-transitory computer readable medium and operable to, when executed:
- i. detect whether a differentially expressed sepsis cell population parameter is present in a heterogenous population of circulating cells by analyzing one or more cell population parameters selected form the group consisting of MO_DC_SD, NE_DC_SD, NE_DC_MEAN, NNRBC_UMALS_SD, MO_ALL_SD, NE_NO, LY_PC, NNRBC_MALS_SD, WNOP, MO_DC_MEAN, WDOP, NNRBC_UMALS_MEAN, BA_PC, NNRBC_MALS_MEAN, BA_PC, NNRBC_MALS_MEAN, EGC_LMALS_MEAN, NNRBC_DC_SD, NNRBC_LMALS_SD, NNRBC_ALL_MEAN, and combinations thereof based at least in part on the light scatter or direct current impedance measurements;
- and
- ii. characterize an inflammatory response to infection based at least in part on the one or more cell population parameters.
A machine comprising:
- a. a transducer module configured to measure at least light scatter and direct current impedance caused by cells passing through a flowcell, wherein the transducer module comprises means for measuring RF conductivity, wherein the means for measuring RF conductivity is operable to measure RF conductivity of cells passing through the flowcell and is also operable to measure RF conductivity of cells passing through a second flowcell comprised by the transducer module;
- and
- b. means for detecting whether a differentially expressed sepsis cell population parameter is present in a heterogenous population of circulating cells and characterizing an inflammatory response to infection based at least in part on that detection.
Dependencies are inferred from the claim text.