Method for determining the antioxidant capacity of a biological sample and related kit
OwnerDeborah PEDONE, Mauro MOGLIANETTI +1inventors
InventorsDeborah PEDONE, Mauro MOGLIANETTI, Pier Paolo POMPA
Life of this patent
Estimating the term…
Abstract
A method for determining the antioxidant power of a sample of a biological fluid or a food is provided. The method involves contacting the sample to be tested with an aqueous solution of palladium nanoparticles, an oxidizing agent, and a chromogenic peroxidase substrate, and detecting the colour of the final solution thus obtained. The colour intensity of the solution is proportional to the antioxidant power of the sample. A kit for carrying out the method is also provided.
Claims18
A method for determining the antioxidant power of a sample of a biological fluid or a food, the method comprising:
- contacting the sample with an aqueous solution of palladium nanoparticles, an oxidizing agent, and a chromogenic peroxidase substrate;
- and
- detecting colour intensity of a final solution thereby obtained, the colour intensity being proportional to the antioxidant power of the sample.
The method of claim1, wherein the food is a fruit juice.
A kit for determining the antioxidant power of a sample of a biological fluid or a food, the kit comprising a chromogenic peroxidase substrate and an aqueous solution of palladium nanoparticles, and optionally further comprising an oxidizing agent and/or a buffer solution, the chromogenic peroxidase substrate being 3,3′,5,5′-tetramethylbenzidine (TMB), the palladium nanoparticles having a diameter ranging from 0.1 nm to 1000 nm, the optional oxidizing agent being hydrogen peroxide and the optional buffer solution being a buffer having a pH comprised between 1 and 7.
An in vitro diagnostic method for assessing oxidative stress in a subject, the in vitro diagnostic method comprising:
- determining the antioxidant power of a biological fluid sample from the subject by contacting the biological fluid sample with an aqueous solution of palladium nanoparticles, an oxidizing agent, and a chromogenic peroxidase substrate, and
- detecting colour intensity of a final solution thereby obtained, the colour intensity being proportional to the antioxidant power of the biological fluid sample, wherein a decreased antioxidant power of the biological fluid sample from the subject compared to a reference sample or value is indicative of the oxidative stress of the subject.
Dependencies are inferred from the claim text.